Levine EMB Agar for microbiology, NutriSelect™ Plus; Synonym: Eosin methylene Blue Lactose Agar according to dipotassium hydrogen phosphate, g/L. Ingredient, Eosin methylene blue (EMB) Agar (g/L). Peptone, 10 g. Lactose, 5 g. Sucrose, 5 g. Dipotassium, PO4, 2 g. Agar, g. Eosin Y, Eosin methylene blue. From Wikipedia, the free encyclopedia. Jump to navigation Jump to search. E. coli on EMB agar. Eosin methylene blue (EMB, also known.
Agar emb l -
Bring to the boil to dissolve completely. Description This versatile medium, modified by Levine1,2, is used for the differentiation of Escherichia coli and Enterobacteria aerogenes, for the rapid identification of Candida albicans, and for the identification of coagulase-positive staphylococci. The medium is prepared to the formula specified by the APHA3,4,5,6 for the detection and differentiation of the coliform group of organisms7,8. Weld 9,10 proposed the use of Levine eosin methylene blue agar, with added chlortetracycline hydrochloride for the rapid identification of Candida albicans in clinical materials.
They found that use of eosin methylene blue agar was just as reliable as more conventional methods for the identification of this organism in sputum. In addition, the medium provided a means for the identification of several Gram- negative genera. Doupagne12 also investigated the use of the Levine medium under tropical conditions. Using the combined rapid technique they were able to obtain results within 48 to 72 hours. Subsequent to the findings of Vogel and Moses 11, Menolasino et al.
The Levine medium was more efficient than tellurite glycine agar and showed good correlation with the plasma coagulase test. Colonial Characteristics Escherichia coli- isolated colonies, mm diameter, with little tendency to confluent growth, exhibiting a greenish metallic sheen by reflected light and dark purple centres by transmitted light.
Enterobacter aerogenes - mm diameter, raised and mucoid colonies, tending to become confluent, metallic sheen usually absent, grey-brown centres by transmitted light. Other Candida species produce smooth yeast-like colonies. Since a typical appearance is variable it is advisable to use a combined method such as that of Walker and Huppert Appearance Dehydrated medium: purple coloured, free-flowing powder Prepared medium: dark purple gel Quality control.